SEQUENCING SERVICES
Capillary Electrophoresis (Sanger Sequencing) will be performed upon request. Samples will have to be provided as per the instructions given below:
- DNA concentration should be measured by Gel estimation and Gel photograph should be attached with clear labelling like sample name and how much µl loaded in each well.
- Data output: Electrophorogram and sequence in text file will be sending through email.
Template Quantity
PCR PRODUCTS
PCR product must be purified & volume should be 10µl.
Plasmids:
Template conc. should be 150-200 ng/µl and minimum volume should be 10µl.
Primer:
Primer conc. shoud be 5-10 pmol/ µl, and minimum volume of 10 µl. Provide 5µl of more primer for every additional reaction.
SPECIAL INSTRUCTIONS
- Please submit samples in 1.5ml micro centrifuge tube.
- Please do not use Tris EDTA buffer for eluting/dissolving your samples.
- Please indicate if your samples have high GC content.
Sequencing – Click here to Download “Sample Request Form for Internal users-IIT Madras“
Sequencing – Click here to Download “Sample Request Form for External users-IIT Madras“