Super-Resolution Microscopy combined with Laser Scanning Confocal Microscope (SR-SIM, PALM and dSTORM)

High Resolution Laser Scanning Confocal microscope LSM 710 combined with ELYRA PS.1 super resolution microscopy system. The super resolution microscopy system integrates with 3D SIM (Structured illumination), PALM (Photo-activated localization microscopy) and STORM (Stochastic Optical Reconstruction microscopy). SR-SIM and d-STORM can provide the ultra-structure details of samples using conventional dyes. PALM is used for endogenously-expressed photo-switchable fluorescent proteins. This system equipped with advanced EM-CCD and sCMOS cameras and wide range of illumination schemes (EPI, HILO, TIRF).

APPLICATION

• Investigate the structural arrangement of a protein.
• Study the arrangement or assembly of multiple proteins.
• Reveal the ultrastructure of cell organelles in 2D/3D.
• Map molecules within a structure.
• Imaging and count molecules in or near the plasma membrane like lipid rafts, receptor clustering or cell-substrate or cell-cell adhesion sites, actin-cytoskeleton remodelling during cell migration.
• Image and quantitative dynamics of proteins using FRAP and FRET.
• Precise localization of proteins, nucleic acid and small molecules within cell compartments.
• Co-localization analysis.
• Dual Color PALM (Fast sequential laser switching to capture two colours simultaneously in PALM).
• Multi-emitter Fitting Algorithms (Allow for denser labelling and enhance acquisition times up to 10-fold in PALM).

Laser Scanning Confocal Microscope LSM 880

High resolution Laser Scanning Confocal Microscope LSM 880 revolutionizes imaging faster than the other regular confocal microscopes. Airyscan was implemented to provide a flexible and robust method to substantially increase resolution and SNR compared with those achieved with a traditional confocal LSM. Airyscan detection module dispenses with the classical physical pinhole and unitary detector assembly and uses a new pinhole-plane image detection assembly based on a novel 32-channel GaAsP-PMT area detector.  The system is equipped with laser lines ranging from UV to visible to near infrared light for multiphoton imaging and live-cell imaging facility.

• To study protein- protein interaction within live cell.
• Measuring simple diffusion of proteins using Fluorescence correlation spectroscopy (FCS/FCCS) in live cells.
• Analyze the order in membranes and other cellular compartments using steady state Fluorescence Anisotropy method.
• Image and quantitative dynamics of proteins using FRAP and FRET.
• Precise localization of proteins, nucleic acid and small molecules within cell compartments.
• Co-localization analysis.

Fully motorized fluorescence microscope Axio Observer Z1 with ApoTome 2 for high quality imaging.The system is equipped with two cameras, CCD and Andor iXon Ultra EMCCD camera for high speed live cell and calcium imaging. The ApoTome 2 (structured illumination) mode allows users to collect images from ‘Z’ stacks, which can subsequently be projected as 3D images and movies.

• Structured illumination images with improved resolution.
• Fast 3D imaging.
• Live cell imaging.
• Co-localization of proteins within cells and tissues.
• Monitor spatio-temporal distributions of small molecules, nucleic acids and proteins within cellular compartment.
• Calcium imaging.
• Cell cycle analysis in real-time.

High content Cytell cell imaging system captures cellular and sub-cellular images with on-board data analysis and visualization tools. Using the system, we can acquire both fluorescence (FITC, DAPI, TRITC, PE) and transmitted light images of cells, colonies and tissues. The system is equipped with 4X and 10X lens. Cells cultured in microplates, slides, Petri dishes and flasks can be imaged using this system. It is a user friendly system equipped with pre-configured, easy-to-use software modules, data acquisition and analysis.

• High throughput cell cycle, apoptosis and cell viability assays.
• Wound healing assays.
• Dye based estimation of Cellular states.

Single or multiple samples (6, 12, 24 and 96 well) with multiple position.